Upstream BRD Technologist - Toronto, Ontario

Job description

Reference Number: R2533558

Position Title: Upstream BRD Technologist (Permanent - Full Time)

Site/Department: Toronto/Global Bioprocess Technology

Major Activities of the Job:

Set up and perform Development studies:

·  Set up experiments and be responsible for performing process development studies to the agreed upon plan. This involves work with laboratory and pilot scale equipment. Often one experiment will involve running several fermenters at one time.
·  Plan the use of equipment, media requirements and inoculum requirements.
·  Carry out the steps in the experiments: Seed culture preparation, preparation of inoculum, preparation of fermenters, inoculation of fermentors, monitor the fermentation sample and control fermenters.
·  The control of fermentors include system for pH dissolve oxygen, temperature, agitation, antifoam and feed stock addition. Fermentors are monitored through sampling and off-line testing, on-line analyses of exhaust gases, and a PC based data collection system. An understanding of these control systems is required.

Operate fermentor and processing equipment under GMP during manufacture of product for clinical use:

·  Understanding of current methodology and SOP’s for fermentation and all general practices involved in GMP operations for manufacture of product for clinical use. Update SOP’s where appropriate.
·  Perform fermentation and clarification processes as part of GMP manufacturing. Raise seed cultures, prepare fermenters, inoculate fermentors, harvest cultures, process cultures and monitor and control equipment.
·  Complete GMP documentation during manufacturing operations: SOP’s, BPR’s and fermentation sheets, record sheets, cleaning logs and maintenance logs.
·  Carry out general GMP duties in work area during manufacturing operations: cleaning and record keeping.
·  Safe disposal of fermentor and purification products, which may include the use of pathogenic organisms.

Harvest and process product from fermentor cultures:

·  Aseptic removal of fermentor culture from the fermenters.
·  Harvest of fermentor cultures by centrifugation or by microfiltration as required.
·  Performing experimental studies to design optimum harvest conditions.
·  Perform experiments: harvest, ultrafiltration and concentration and/or centrifugation, homogenization, clarification, sample and submit for testing.
·  Chemical extraction of fermentor products as required.

Set up and perform tests:

·  Perform assays that are applicable to development fermentation or clinical manufacturing operations in progress. This will include optical density, dry weight, microbiological staining, planting for cell viability (colony forming units), protein determination, SDS-PAGE and other biochemical assays that may be required.
·  Requires preparation of appropriate reagents and calibration of equipment.
·  Sample preparation of materials to be sent to other departments (e.g. QC) or for outside testing.

Analysis and documentation of all results:

·  Parameters, procedures, data and results are recorded neatly and accurately in a laboratory book. Collected data is logged onto computer in spreadsheet format. Results and data are analyzed by calculation and graphing.
·  Complete GMP documentation during manufacturing processes; BPR’s record sheets, cleaning logs and maintenance logs.

Cleaning and Maintenance of equipment:

·  Cleaning of fermentor vessels, pumps, homogenizer, filtration equipment and accessories.
·  Routine manipulation of fermentors involves replacement of pH probes and DO probe membranes, inspection and replacement of O-ring seals and calibration of pumps.
·  Routine maintenance of homogenizer involves dismantling it and replaces spring, O-ring seals keep ceramic free of debris.

No employee has a proprietary right to an activity or group of activities. Activities may be changed or reassigned to meet the needs of the operations.

Minimum Qualifications:

·  Minimum four (4) years Bachelor’s Degree (Microbiology, Biochemistry), plus a minimum one (1) year of previous relevant work experience.
·  Working knowledge in the following areas: Microbiological techniques and analysis and Biochemical techniques
·  Strong Computer, Mechanical and General Lab skills
·  Excellent written and verbal communication skills.
·  Ability to co-ordinate several simultaneous activities with shared equipment and workplace
·  Ability to work independently and as part of a team

Applications received after the official closing date will be reviewed on an individual basis.

Hours Of Work:

Inclusive from Monday to Friday 8:00a.m. to 4:00p.m.

This is subject to change at any time and it should not be construed as limiting the right of the company to make such change to meet the requirements of the operation.

Sanofi Pasteur: The world’s leading vaccine company

Sanofi Pasteur, the vaccines division of Sanofi, is the largest company in the world devoted entirely to human vaccines. Our driving goal is to protect people from infectious diseases by creating safe and effective vaccines.  Our company distributes more than 1 billion doses of vaccine each year, making it possible to vaccinate more than 500 million people across the globe.  Sanofi Pasteur offers the broadest range of vaccines in the world, providing protection against 20 bacterial and viral diseases.

Sanofi is dedicated to supporting people through their health challenges. We are a global biopharmaceutical company focused on human health. We prevent illness with vaccines, provide innovative treatments to fight pain and ease suffering. We stand by the few who suffer from rare diseases and the millions with long-term chronic conditions.  With more than 100,000 people in 100 countries, Sanofi is transforming scientific innovation into healthcare solutions around the globe.

Sanofi is an equal opportunity employer committed to diversity and inclusion. Our goal is to attract, develop and retain highly talented
employees from diverse backgrounds, allowing us to benefit from a wide variety of experiences and perspectives. We welcome and encourage applications from all qualified applicants.  Accommodations for persons with disabilities required during the recruitment process are available upon request.

Thank you in advance for your interest.

Only those candidates selected for interviews will be contacted.

Sanofi, Empowering Life

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At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all.