Scientist, Downstream-Integrated Biologics Profiling

Job description

Job Description

Responsible for protein purification at lab-scale from CHO expression system to supply API (Active Pharmaceutical Ingredient) with Low Endotoxin levels for developability assessment and pre-clinical studies. Contribute to developability assessment on Novartis biologics pipeline to select lead candidate for clinical development. Perform evaluation of downstream process to identify manufacturability challenges for clinical development. In particular, develop and execute lab-scale purification processes using various chromatography techniques such as AC (Affinity), AEC (Anion Exchange), CEC (Cation Exchange), HIC (Hydrophobic Interaction), SEC (Size Exclusion) and Mixed Mode, and filtration techniques such as UF/DF (Ultrafiltration/Diafiltration) for the production of antibodies and therapeutic proteins to meet internal and external material requests for drug discovery. Work on various lab equipment including, but not limited to AKTA Pilot, AKTA avant, AKTA Explorer, AKTA Pure, SciLog systems, Bench and Pilot TFF (Tangential Flow Filtration), Nanodrop and Centrifuge. Contribute to cross-functional team responsible for profiling and production of protein drug substance. Support several projects and manage accelerated project timelines. Perform documentation, complex sample handling and data tracking as required. Coordinate material supply, logistics, and production timelines. Write and review various LPs (Lab Procedures) to develop purification strategies. Work closely with analytical team to coordinate all in-process analytics for decision-making on suitable production process. Analyze data of various assays such as SEC-HPLC (Size Exclusion- High Performance Liquid Chromatography), AC- HPLC (Affinity- High Performance Liquid Chromatography), LabChip, MS (Mass Spectrometry), UV/VIS, Nanodrop, SDS-PAGE (sodium dodecyl sulfate- polyacrylamide gel electrophoresis). Accurately and timely process all data, prepare summary documents, write technical reports and present in cross-functional project teams.

EEO Statement

The Novartis Group of Companies are Equal Opportunity Employers and take pride in maintaining a diverse environment. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race, color, religion, gender, national origin, age, sexual orientation, marital or veteran status, disability, or any other legally protected status.

Desired profile

Minimum requirements

Scientist, Downstream-Integrated Biologics Profiling sought by Novartis Institutes for Biomedical Research, Inc. for Cambridge MA location. Must possess Master’s degree in Biological Sciences, Biochemistry, or Biochemical/Chemical Engineering, and minimum 2 years industry experience in protein purification using various chromatography techniques such as AC, AEC, CEC, HIC, SEC and Mixed Mode, and filtration techniques (UF/DF/TFF) for biopharmaceuticals research. Experience must include AKTA connectivity and troubleshooting; in-process analytics such as Nanodrop, UV/VIS, SDS-PAGE, AC-HPLC and SEC-HPLC; data analysis and technical report writing; and demonstrated success working in cross-functional teams, performing documentation, complex sample handling, and data tracking. Respond to: NIBRI, Attn: HR/Perm, Req 203525BR, 200 Technology Sq, 10th Fl, Cambridge MA 02139 or online www.novartis.com/careers. Novartis is an EOE.